Cellular and molecular characteristics of epithelial Na+ channels. Recent work has focused on the defining the subunit stoichiometry of the Na+ channel, characterizing the structure of the channel’s pore, and on the identification of sites within Na+ channel subunits that facilitate subunit interactions and assembly.
Regulation of epithelial Na+ channels. We are examining mechanisms by which selected hormones regulate epithelial Na+ channels. Recent studies have focused on the regulation of epithelial Na+ channels by mechanical forces and by the hormone aldosterone. We have initiated studies directed at identifying K+ channels in renal collecting tubules that are regulated by mechanical forces.
Functional interactions between epithelial Na+ channels and cystic fibrosis transmembrane conductance regulator (CFTR) Cl- channels. We are examining mechanisms by which Na+ channels are functionally altered in cystic fibrosis.